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Catalog Number | orb7089 |
---|---|
Category | Antibodies |
Description | Phospho-P38 MAPK (Thr180) Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IF, IHC-Fr, IHC-P, WB |
Predicted Reactivity | Canine, Equine, Gallus, Porcine, Rabbit |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated Synthesised phosphopeptide derived from human MAPK14 around the phosphorylation site of Thr180 EM(p-T)G |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 41 kDa |
Target | MAPK14 |
UniProt ID | Q16539 |
RRID | AB_10940307 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | p38(phospho T180); p-p38(phospho T180); MAPK14(pho Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Blank control (blue): Hela (fixed with 2% paraformaldehyde (10 min), then permeabilized with 0.3% tritionx-100 for 5 min at room temperature). Primary Antibody: Rabbit Anti-phospho-P38 MAPK (Thr180) antibody (orb7089) Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC), dilution: 1:200 in 1X PBS containing 0.5% BSA.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (p-P38 MAPK (Thr180)) Polyclonal Antibody, Unconjugated (orb7089) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-P38 MAPK (Thr180)) Polyclonal Antibody, Unconjugated (orb7089) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (p-P38 MAPK (Thr180)) Polyclonal Antibody, Unconjugated (orb7089) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Sample: Muscle (Mouse) Lysate at 40 ug, Muscle (Rat) Lysate at 40 ug, Primary: Anti-phospho-P38 MAPK (Thr180) (orb7089) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 41 kD.
ELISA, FC, ICC, IF, IHC-Fr, IHC-P, WB | |
Canine, Equine, Gallus, Human, Porcine, Rabbit | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-Fr, IHC-P, WB | |
Canine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-Fr, IHC-P, WB | |
Canine, Equine, Gallus, Human, Porcine, Rabbit | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
HRP |
FC, IF | |
Canine, Equine, Gallus, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
PE/Cy7 |
FC, IF | |
Canine, Equine, Gallus, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
PE/Cy5.5 |