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Catalog Number | orb7090 |
---|---|
Category | Antibodies |
Description | Phospho-p38 MAPK (Tyr323) Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-Fr, IHC-P, WB |
Predicted Reactivity | Canine, Equine, Gallus, Porcine, Rabbit, Rat |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | KLH conjugated Synthesised phosphopeptide derived from human MAPK14 around the phosphorylation site of Tyr323 DP(p-Y)DQ |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 41 kDa |
Target | MAPK14 |
UniProt ID | Q16539 |
RRID | AB_10961398 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | MAPK14(phospho T323); CSAID Binding Protein 1; CSA Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Zhao, Haiyan et al. Inhibition of endocan attenuates monocrotaline-induced connective tissue disease related pulmonary arterial hypertension Int Immunopharmacol, 42, 115-121 (2017)
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-p38 MAPK (Tyr323)) Polyclonal Antibody, Unconjugated (orb7090) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse esophagus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-p38 MAPK (Tyr323)) Polyclonal Antibody, Unconjugated (orb7090) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-p38 MAPK (Tyr323)) Polyclonal Antibody, Unconjugated (orb7090) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Brain (Mouse) Lysate at 40 ug, Primary: Anti-phospho-p38 MAPK (Tyr323) (orb7090) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 45 kD.
IF, IHC-Fr, IHC-P | |
Canine, Equine, Gallus, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF | |
Canine, Equine, Gallus, Porcine, Rabbit, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
PE/Cy7 |
IF | |
Canine, Equine, Gallus, Porcine, Rabbit, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
PE/Cy5.5 |
IF | |
Canine, Equine, Gallus, Porcine, Rabbit, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
APC |
IF | |
Canine, Equine, Gallus, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
APC |