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Phospho-p38 MAPK (Tyr323) Rabbit Polyclonal Antibody

Catalog Number: orb7090

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb7090
CategoryAntibodies
DescriptionPhospho-p38 MAPK (Tyr323) Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityCanine, Equine, Gallus, Porcine, Rabbit, Rat
ReactivityHuman, Mouse
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human MAPK14 around the phosphorylation site of Tyr323 DP(p-Y)DQ
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW41 kDa
TargetMAPK14
UniProt IDQ16539
RRIDAB_10961398
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesMAPK14(phospho T323); CSAID Binding Protein 1; CSA
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NoteFor research use only
Expiration Date12 months from date of receipt.
Phospho-p38 MAPK (Tyr323) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-p38 MAPK (Tyr323)) Polyclonal Antibody, Unconjugated (orb7090) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-p38 MAPK (Tyr323) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse esophagus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-p38 MAPK (Tyr323)) Polyclonal Antibody, Unconjugated (orb7090) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-p38 MAPK (Tyr323) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-p38 MAPK (Tyr323)) Polyclonal Antibody, Unconjugated (orb7090) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-p38 MAPK (Tyr323) Rabbit Polyclonal Antibody

Sample: Brain (Mouse) Lysate at 40 ug, Primary: Anti-phospho-p38 MAPK (Tyr323) (orb7090) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 45 kD.