MAP4K1 Rabbit Polyclonal Antibody

• Catalog Number: orb6373
• Category: Antibodies
• Description: MAP4K1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Mouse, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MAP4K1 (231-330/833aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 91 kDa
• Target: MAP4K1
• UniProt ID: Q92918
• RRID: AB_10925959
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Hematopoietic progenitor kinase 1; Hematopoietic p
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Hep G2 Cells (blue), fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Primary Antibody: Rabbit Anti-MAP4K1/AF647 Conjugated antibody (orb6373/AF647), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF647 (orange), used under the same conditions.

Sample: U937 (Human) Cell Lysate at 30 ug, Primary: Anti-MAP4K1 (orb6373) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 91 kD, Observed band size: 91 kD.