MAP4K1 Antibody

• Catalog Number: orb1925392
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB58112
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: WB: 1:2000, WB: 1:2000, IHC-P-Leica: 1:500, IHC-P-Leica: 1:500
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 91296 Da
• Target: This MAP4K1 antibody is generated from a rabbit immunized with a recombinant protein from human MAP4K1.
• UniProt ID: Q92918
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Mitogen-activated protein kinase kinase kinase kin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-MAP4K1 Antibody at 1:2000 dilution + Raji whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 91 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue was performed on the Leica BOND RXm. Samples were incubated with primary antibody (1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded human epityphlon tissue was performed on the Leica BOND RXm. Samples were incubated with primary antibody (1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-MAP4K1 Antibody at 1:2000 dilution. Lane 1: Daudi whole cell lysate. Lane 2: Raji whole cell lysate. Lane 3: Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 91 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.