Goat anti-HPK1 / MAP4K1 Antibody

• Catalog Number: orb18655
• Category: Antibodies
• Description: Goat polyclonal antibody to MAP4K1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:32000, WB: 0.5-2 μg/ml, IHC-P: 2.5 μg/ml
• Conjugation: Unconjugated
• MW: 90.4; 91.3
• Target: HPK1 / MAP4K1
• Entrez: 11184
• Protein Sequence: DVVDPDIFNRDPR
• RRID: AB_10748628
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti MAP4K1 antibody, anti mitogen-activated prote
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 95-100kDa band observed in Jurkat (predicted MW of 96kDa according to NP_009112). Recommended for use at 0.5-2 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18655 staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemical staining of Human Thymus using MAP4K1 antibody