LC3B Antibody, KO Validated

• Catalog Number: orb1274430
• Category: Antibodies
• Description: LC3B Antibody, KO Validated
• Target: MAP1LC3B
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: A synthetic peptide of human LC3B
• UniProt ID: Q9GZQ8
• MW: Observed: 14kDa, 16kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MAP1LC3B Antibody: LC3B, ATG8F, MAP1LC3B-a, MAP1A/
• Research Area: Cancer Biology, Cell Biology, Neuroscience, Signal
• Note: For research use only

Western blot analysis of extracts of various cell lines, using LC3B antibody (orb1274430) at 1:1000 dilution. C6 cells were treated by Chloroquine (50 μM) for 20 hours. Hela cells were treated by Chloroquine (50 μM) for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 μM) for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Western blot analysis of extracts of pig liver, using LC3B antibody (orb1274430). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts from normal (control) and LC3B knockout (KO) 293T cells, using LC3B antibody (orb1274430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat heart using LC3B antibody (orb1274430) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using LC3B antibody (orb1274430) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using LC3B antibody (orb1274430) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using LC3B antibody (orb1274430) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using LC3B antibody (orb1274430) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using LC3B antibody (orb1274430) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using HIRIP3 antibody (orb1256659) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using LC3B antibody (orb1274430) at dilution of 1:100. Hela cells were treated by Chloroquine (50 μM) for 20 hours (left). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using LC3B antibody (orb1274430) at dilution of 1:100. NIH/3T3 cells were treated by Chloroquine (50 μM) for 20 hours (left). Blue: DAPI for nuclear staining.