Cleaved LC3A Antibody

SKU: orb33336

Description

Rabbit polyclonal antibody to Cleaved LC3

Research Area

Cell Biology

Images & Validation

• Tested Applications: ICC, IF, IHC-P, WB
• Dilution Range: IF - 1:25, WB - 1:500, IHC-P - 1:25, ICC - 1:10-50
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat, Zebrafish

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB38908
• Immunogen: Synthetic Peptide
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ATG8F antibody, anti Autophagy-related protein LC3 B antibody, anti Autophagy-related ubiquitin-like modifier LC3 B antibody, anti LC3 antibody, anti LC 3 antibody, anti LC3A antibody, anti LC3B antibody, anti LC3II antibody, anti MAP1 light chain 3 like protein 2 antibody, anti MAP1 light chain 3-like protein 2 antibody, anti MAP1A/1BLC3 antibody, anti MAP1A/MAP1B LC3 B antibody, anti MAP1A/MAP1B light chain 3 B antibody, anti MAP1ALC3 antibody, anti MAP1LC3B a antibody, anti Map1lc3b antibody, anti Microtubule associated protein 1 light chain 3 beta antibody, anti Microtubule-associated protein 1 light chain 3 beta antibody, anti Microtubule-associated proteins 1A/1B light chain 3B antibody

Western blot analysis of anti-cleaved-LC3 (APG8a) Pab in mouse brain tissue lysate. Cleaved-LC3 (APG8a) was detected using the purified Pab.

Western blot analysis of lysates from NIH/3T3 cells, untreated or treated with chloroquine, using Cleaved-APG8a (MAP1LC3A) (upper) or Beta-actin (lower).

Western blot analysis of lysates from NIH/3T3 cells, untreated or treated with chloroquine, using Cleaved-APG8a (MAP1LC3A) (upper) or Beta-actin (lower).

Western blot analysis of lysates from A431 cell line, untreated or treated with chloroquine, 100ng/ml, using Cleaved-APG8a (MAP1LC3A) (upper) or Beta-actin (lower).

Western blot analysis of lysates from A431 cell line, untreated or treated with chloroquine, 100ng/ml, using Cleaved-APG8a (MAP1LC3A) Antibody (upper) or Beta-actin (lower).

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse mouse embryonic fibroblasts cell line) cells labeling MAP1LC3A at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody at 1/400 dilution (green). The nuclear counter stain is DAPI (blue). Immunofluorescence image showing cytoplasm on NIH/3T3 cell line.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line cells labeling MAP1LC3A at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody at 1/400 dilution (green). Immunofluorescence image showing vesicles staining on Hela cell line.The nuclear counter stain is DAPI (blue). The right image is Hela cells treated with Chloroquine 50μM for 16 h.

Quick Database Links

UniProt

Q9GZQ8, Q9H492

RefSeq (Protein):NP_852610.1, NP_115903.1

Gopinath Mukherjee et al. TREATMENT OF MICE WITH TLR4 AND IFN-γ ANTIBODY AND EXOGENOUS IL-10 MODULATES BCL2 AND LC3 EXPRESSION IN RANKL/M-CSF PLUS LPS STIMULATED BONE MARROW MACROPHAGES-LEADING TO BONE RESORPTION researchgate.net, (2025)

PubMed:

Applications

WB

Reactivity

Mouse