APG8b(MAP1LC3B) Antibody (N-term T29)

SKU: orb1937429

Description

APG8b(MAP1LC3B) Antibody (N-term T29)

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution range: IHC-P-Leica - 1:500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 14688 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

MAP1ALC3

APG8b (MAP1LC3B) Antibody (N-term T29) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

APG8b (MAP1LC3B) Antibody (T29) western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the APG8b (MAP1LC3B) antibody detected the APG8b (MAP1LC3B) protein (arrow).

APG8b (MAP1LC3B) Antibody (T29) western blot analysis in mouse NIH-3T3 cell line lysates (35 ug/lane). This demonstrates the APG8b (MAP1LC3B) antibody detected the APG8b (MAP1LC3B) protein (arrow).

Immunohistochemical analysis of paraffin-embedded human brain tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Immunohistochemical analysis of paraffin-embedded human liver tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Quick Database Links

UniProt

Q9GZQ8