You have no items in your shopping cart.
Cart summary
Item 1 of 5
Item 1 of 5
APG8b(MAP1LC3B) Antibody (N-term T29)
Catalog Number: orb1937429
| Catalog Number | orb1937429 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB28014 |
| Tested applications | FC, IHC-P, WB |
| Predicted Reactivity | Rat |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, IHC-P-Leica: 1:500, IHC-P-Leica: 1:500, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 14688 Da |
| Target | This APG8b(MAP1LC3B) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-33 amino acids from the N-terminal region of human APG8b(MAP1LC3B). |
| UniProt ID | Q9GZQ8 |
| NCBI | NP_073729.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Microtubule-associated proteins 1A/1B light chain Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
APG8b (MAP1LC3B) Antibody (N-term T29) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
APG8b (MAP1LC3B) Antibody (T29) western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the APG8b (MAP1LC3B) antibody detected the APG8b (MAP1LC3B) protein (arrow).
APG8b (MAP1LC3B) Antibody (T29) western blot analysis in mouse NIH-3T3 cell line lysates (35 ug/lane). This demonstrates the APG8b (MAP1LC3B) antibody detected the APG8b (MAP1LC3B) protein (arrow).
Immunohistochemical analysis of paraffin-embedded human brain tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.
Immunohistochemical analysis of paraffin-embedded human liver tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.
