LC3 Antibody

• Catalog Number: orb628393
• Category: Antibodies
• Description: Rabbit polyclonal antibody to LC3
• Target: LC3
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: liquid
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Immunogen affinity purified
• Immunogen: microtubule-associated protein 1 light chain 3 beta
• UniProt ID: Q9GZQ8
• MW: 16 kDa
• Tested applications: ELISA, IF, IHC, IP, WB
• Dilution range: WB: 1:500-1:2000; IP: 1:500-1:2000 ; IHC: 1:20-1:200; IF: 1:20-1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: LC3, LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/M
• Research Area: Autophagic, Cancer, Epigenetics, Neuroscience, Sig
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Chloroquine treated HeLa cells were subjected to SDS PAGE followed by western blot with orb628393 (LC3 Antibody) at dilution of 1:1000.

IP Result of anti-LC3 (IP: orb628393, 3 ug; Detection: orb628393 1:1000) with mouse brain tissue lysate 4000 ug.

Immunohistochemistry of paraffin-embedded human gliomas using orb628393 (LC3 antibody) at dilution of 1:50.