LC3 Rabbit Polyclonal Antibody

SKU: orb628393

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Description

Rabbit polyclonal antibody to LC3

Research Area

Autophagic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IF, IHC, IP, WB
Dilution Range	WB: 1:500-1:2000; IP: 1:500-1:2000 ; IHC: 1:20-1:200; IF: 1:20-1:200
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	microtubule-associated protein 1 light chain 3 beta
Target	LC3
Molecular Weight	16 kDa
Purity	≥95% as determined by SDS-PAGE
Purification	Immunogen affinity purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	liquid
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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LC3, LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3, MAP1LC3B

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Chloroquine treated HeLa cells were subjected to SDS PAGE followed by western blot with orb628393 (LC3 Antibody) at dilution of 1:1000.

IP Result of anti-LC3 (IP: orb628393, 3 ug; Detection: orb628393 1:1000) with mouse brain tissue lysate 4000 ug.

Immunohistochemistry of paraffin-embedded human gliomas using orb628393 (LC3 antibody) at dilution of 1:50.

Quick Database Links

Gene Symbol

LC3

UniProt

Q9GZQ8

UniProt Details

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No UniProt data available

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Datasheet

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Immunoprecipitation

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