ITK Antibody

• Catalog Number: orb1248351
• Category: Antibodies
• Description: ITK Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Rat
• Immunogen: The immunogen for this antibody is: C-KERPEDRPAFSR
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 70kDa
• Target: ITK
• UniProt ID: Q08881
• NCBI: NP_005537.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ITK, IL2-inducible T-cell kinase, EMT, LYK, MGC126
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 70kDa band observed in Rat Thymus lysates (calculated MW of 72.4kDa according to Rat NP_001102295.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248351 (3.75 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248351 (0.1 ug/ml) staining of Rat Thymus lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.