ITK Antibody

SKU: orb1248351

Description

ITK Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 70kDa band observed in Rat Thymus lysates (calculated MW of 72.4kDa according to Rat NP_001102295.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-KERPEDRPAFSR
• Target: ITK
• Molecular Weight: Approx 70kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

ITK, IL2-inducible T-cell kinase, EMT, LYK, MGC126257, MGC126258, PSCTK2, homolog of mouse T-cell itk/tsk, tyrosine-protein kinase ITK/TSK, tyrosine-protein kinase LYK

orb1248351 (3.75 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248351 (0.1 ug/ml) staining of Rat Thymus lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

ITK

UniProt

Q08881

RefSeq (Protein):NP_005537.3