ITK Antibody

SKU: orb137166

Description

Goat polyclonal to ITK

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 3.75ug/ml
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine
• Application Notes:
  WB: Approx 70kDa band observed in Rat Colon, Lung and Spleen lysates (calculated MW of 72.4kDa according to Rat NP_001102295.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.. IHC: Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of Jurkat cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concen

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ITK
• Molecular Weight: 71.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti ITK antibody

Western blot analysis of Rat Thymus lysate using ITK antibody

Immunohistochemical staining of Human Spleen using ITK antibody

Flow cytometric analysis of paraformaldehyde fixed Jurkat cells using ITK antibody.

Quick Database Links

Gene Symbol

ITK

NCBI Gene ID

3702