ITK Antibody

• Catalog Number: orb137166
• Category: Antibodies
• Description: Goat polyclonal to ITK
• Target: ITK
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KERPEDRPAFSR
• MW: 71.8
• Tested applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 3.75ug/ml
• Application notes: WB: Approx 70kDa band observed in Rat Colon, Lung and Spleen lysates (calculated MW of 72.4kDa according to Rat NP_001102295.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.. IHC: Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of Jurkat cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concen
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti ITK antibody
• Note: For research use only
• Entrez: 3702
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Rat Thymus lysate using ITK antibody

Immunohistochemical staining of Human Spleen using ITK antibody

Flow cytometric analysis of paraformaldehyde fixed Jurkat cells using ITK antibody.