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ITK Antibody
Description
Images & Validation
−| Tested Applications | FC, ICC, WB |
|---|---|
| Dilution range | WB: 1/500 - 1/2000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 5G6A6 |
| Immunogen | Purified recombinant fragment of human ITK expressed in E. Coli. |
| Target | ITK |
| Molecular Weight | 72kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Disclaimer | For research use only |
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Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of ITK antibody

Western blot analysis of human ITK (AA: 2-120) recombinant protein using ITK antibody

Confocal immunofluorescence analysis of Jurkat cells using ITK antibody

Flow cytometric analysis of Jurkat cells using ITK antibody

Western blot analysis of Jurkat cell lysate using ITK antibody
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Protocol Information
ITK Antibody (orb97350)
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