ITK Antibody

• Catalog Number: orb18315
• Category: Antibodies
• Description: Goat polyclonal antibody to ITK
• Target: ITK
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: RLLRQLAEIAESGL
• RRID: AB_10749738
• MW: 71.8
• Tested applications: ELISA, IF, WB
• Dilution range: ELISA: 1:2000, WB: 1-3g/ml, IHC-P: 10 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 75 kDa bands seen in Jurkat Lysates. Recommended for use at 0.5-2 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti ITK antibody, anti EMT antibody, anti LYK ant
• Research Area: Epigenetics, Immunology
• Note: For research use only
• Entrez: 3702
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Jurkat nuclear cell lysate (A) and negative control Human parathyroid gland (B) using ITK antibody.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using ITK antibody.

Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells using ITK antibody.