IL10 Antibody

• Catalog Number: orb1258241
• Category: Antibodies
• Description: IL10 Antibody
• Target: IL10
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 19-178 of human IL10 (NP_000563.1).
• UniProt ID: P22301
• MW: Observed: 22kDa
• Tested applications: IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Interleukin 10Interleukin-10, Cytokine synthesis i
• Research Area: Cancer Biology, Cell Biology, Immunology & Inflamm
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of Jurkat cells, using IL10 antibody (orb1258241) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded mouse spleen using IL10 antibody (orb1258241) at dilution of 1:200 (40x lens).