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IL-10 Rabbit Polyclonal Antibody

Catalog Number: orb100193

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb100193
CategoryAntibodies
DescriptionIL-10 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityGallus, Guinea pig, Porcine, Rabbit, Rat, Sheep
ReactivityHuman, Mouse
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human IL-10 (101-178/178aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW18 kDa
TargetIL10
UniProt IDP22301
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesInterleukin-10 precursor; Cytokine synthesis inhib
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NoteFor research use only
Expiration Date12 months from date of receipt.

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Applications

IHC

Reactivity

Human

Dambergs, Kristaps et al. Morphopathogenesis of Adult Acquired Cholesteatoma Medicina (Kaunas), 59, 306 (2023)

Applications

IHC

Reactivity

Human
IL-10 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IL-10) Polyclonal Antibody, Unconjugated (orb100193) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

IL-10 Rabbit Polyclonal Antibody

Tissue/cell: human glioma tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-IL-10 Polyclonal Antibody, Unconjugated (orb100193) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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    200 μl, 100 μl, 50 μl
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    Mouse, Rat

    Human, Mouse, Rat

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    Unconjugated

    100 μl, 200 μl, 50 μl
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    IF,  IHC-Fr,  IHC-P,  WB

    Gallus, Guinea pig, Porcine, Rabbit, Rat, Sheep

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 200 μl, 50 μl
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    ELISA,  IHC-P,  WB

    Human, Mouse, Rat

    Polyclonal

    Unconjugated

    100 μl, 50 μl