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IL-10 Rabbit Polyclonal Antibody

Catalog Number: orb10892

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb10892
CategoryAntibodies
DescriptionIL-10 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityGallus, Guinea pig, Porcine, Rabbit, Rat, Sheep
ReactivityHuman, Mouse
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human IL-10 (101-178/178aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW21 kDa
TargetIL10
UniProt IDP22301
RRIDAB_10751973
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesInterleukin-10 precursor; Cytokine synthesis inhib
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NoteFor research use only
Expiration Date12 months from date of receipt.
IL-10 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human lung cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IL-10) Polyclonal Antibody, Unconjugated (orb10892) at 1:200 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

IL-10 Rabbit Polyclonal Antibody

Sample: Lane 1: Recombinant human IL-10 protein, His (HEK293), Primary: Anti-IL-10 (orb10892) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 18 kDa, Observed band size: 21 kDa.

IL-10 Rabbit Polyclonal Antibody

Tissue/Cell: mouse kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IL-10 Polyclonal Antibody, Unconjugated (orb10892) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

  • IL-10 Rabbit Polyclonal Antibody [orb100193]

    IF,  IHC-Fr,  IHC-P,  WB

    Gallus, Guinea pig, Porcine, Rabbit, Rat, Sheep

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl, 200 μl
  • IL-10 Polyclonal Antibody [orb1411632]

    IHC-P,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • IL-22 Rabbit Polyclonal Antibody [orb5549]

    FC,  IF,  IHC-Fr,  IHC-P,  WB

    Canine, Rat

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 100 μl, 50 μl
  • IL-10 Rabbit Polyclonal Antibody [orb317642]

    WB

    Mouse, Rat

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 200 μl, 50 μl
  • IL-10 rabbit pAb [orb766743]

    ELISA,  IHC-P,  WB

    Human, Mouse, Rat

    Polyclonal

    Unconjugated

    100 μl, 50 μl