IL-10 Rabbit Polyclonal Antibody

SKU: orb10892

Description

IL-10 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Gallus, Guinea pig, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IL-10 (101-178/178aa)
• Target: IL10
• Molecular Weight: 21 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Interleukin-10 precursor; Cytokine synthesis inhibitory factor; CSIF; Cytokine Synthesis Inhibitory Factor; IL 10; IL10A; Interleukin 10; Interleukin10; MGC126450; MGC126451; TGIF; IL10_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (Human lung cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IL-10) Polyclonal Antibody, Unconjugated (orb10892) at 1:200 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Recombinant human IL-10 protein, His (HEK293), Primary: Anti-IL-10 (orb10892) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 18 kDa, Observed band size: 21 kDa.

Tissue/Cell: mouse kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IL-10 Polyclonal Antibody, Unconjugated (orb10892) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

IL10

UniProt

P22301

References

Z Hajimashhadi, F Imani, F Nasirinezhad Intrathecal Administration of [Pyr1] apelin-13 Improves Functional Recovery in Rat Via Reducing Astrocytic Induced Inflammation in Ventral and Dorsal Spinal Cord International Journal of Peptide Research and Therapeutics,, (2025)

PubMed:

Applications

WB

Reactivity

Rat

Narges Maleki 1, Maryam Rezapour Kalkhorann 1, Mohammad Sajad Sajad Emami Aleagha 2, Amir Emami 3, Abdolamir Allameh MSCs engineered with secreted Klotho alleviate blood-brain barrier disruption and reduce neuroinflammation more effectively than MSCs in experimental autoimmune encephalomyelitis Stem Cell Res Ther ., (2025)

PubMed: 40598580