IL-10 Rabbit Polyclonal Antibody

• Catalog Number: orb317642
• Category: Antibodies
• Description: IL-10 Rabbit Polyclonal Antibody
• Target: IL10
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human IL-10 (51-100/178aa)
• UniProt ID: P22301
• MW: 20 kDa
• Tested applications: WB
• Dilution range: WB=1:500-2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Interleukin-10 precursor; Cytokine synthesis inhib
• Research Area: Cell Biology, Immunology, Interleukins
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Recombinant human IL-10 protein, His (HEK293), Primary: Anti-IL-10 (orb317642) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 20 kDa, Observed band size: 17 kDa.

Sample: Plasma (Rat) at 40 ug, Plasma (Rat) at 40 ug, Primary: Anti-IL-10 (orb317642) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 20 kD, Observed band size: 20 kD.

Sample: Raw264.7 (Mouse) Lysate at 30 ug, Primary: Anti-IL-10 (orb317642) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 20 kD, Observed band size: 20 kD.