IL-10 Antibody

• Catalog Number: orb1538986
• Category: Antibodies
• Description: IL-10 Antibody
• Target: IL-10
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Lyophilized from PBS, pH 7.2. Lyophilized from PBS, pH 7.2
• Purification: Immunoaffinity purified
• Immunogen: E.coli derived recombinant Human IL-10.
• Tested applications: ELISA, IHC, IHC-P, WB
• Dilution range: ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml)
• Application notes: Resuspension: Reconstitute in sterile water to 0.1-1 mg/ml. . Further information: Immunohistochemistry: LS-B7432 in an antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-10 (1 ng/ml), a concentration of 1.2-1.8 ug/ml of this antibody is required. ELISA: To detect hIL-10 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-10 (LS-C104321) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-10. Western Blot: To detect hIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-10 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IL10, CSIF, GVHDS, IL-10, IL10A, Interleukin-10, I
• Note: For research use only