IL-10 Antibody

• Catalog Number: orb1272493
• Category: Antibodies
• Description: IL-10 Antibody
• Target: Il10
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Rat
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Purification: Anti-rIL-10 specific antibody was purified by affinity chromatography employing immobilized rIL-10 matrix.
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rIL-10 (rat Interleukin-10).
• UniProt ID: P29456
• Tested applications: ELISA, NeA, WB
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IL10X, Il-10, Interleukin-10, Cytokine synthesis i
• Research Area: Immunology & Inflammation
• Note: For research use only
• NCBI: P29456
• Expiration Date: 12 months from date of receipt.

To detect Rat IL-10 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Rat IL-10 (orb1272492) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-10.

To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.