IL-10 Antibody [2F4-11-1]

• Catalog Number: orb1272608
• Category: Antibodies
• Description: IL-10 Antibody [2F4-11-1]
• Target: IL10
• Clonality: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Rat
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Purification: This antibody was purified from tissue culture by Protein A affinity chromatography.
• Immunogen: E.coli derived Recombinant Rat IL-10
• UniProt ID: P22301
• Tested applications: ELISA, WB
• Application notes: ELISA:Sandwich:In a sandwich ELISA (assuming 100μL/well), a concentration of 8.0-16.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant Rat IL-10 when used with ProSci’s Polyclonal Biotinylated Antigen Affinity Purified Anti-Rat IL-10 (XP-5163Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot:To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-10 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
• Antibody Type: Primary Antibody
• Clone Number: 2F4-11-1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Interleukin 10Interleukin-10, Cytokine synthesis i
• Research Area: Cancer Biology, Infectious Disease & Virology, Neu
• Note: For research use only
• NCBI: NP_000563.1

To a sandwich ELISA (assuming 100 ul/well), a concentration of 8.0-16.0 ug/ml of this antibody will detect at least 500 pg/ml of recombinant Rat IL-10 when used with Biotinylated Antigen Affinity Purified Anti-Rat IL-10 (orb1272492) as the detection antibody at a concentration of approximately 0.5-1.0 ug/ml.

To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-10 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.

To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-10 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.