IL-10 Antibody [15B9-1-2]

• Catalog Number: orb1272609
• Category: Antibodies
• Description: IL-10 Antibody [15B9-1-2]
• Target: IL10
• Clonality: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Purification: This IgG2A antibody was purified from cell culture by Protein G affinity chromatography.
• Immunogen: E.coli derived Recombinant Murine IL-10
• UniProt ID: P22301
• Tested applications: ELISA, NeA, WB
• Application notes: ELISA:Sandwich:In a sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 1600 pg/mL of recombinant murine IL-10 when used with ProSci’s Polyclonal biotinylated antigen affinity purified anti-murine IL-10 as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot:To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of mIL-10 (0.5 ng/mL), a concentration of 3.0-5.0 μg/mL of this antibody is required.
• Antibody Type: Primary Antibody
• Clone Number: 15B9-1-2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Interleukin 10Interleukin-10, Cytokine synthesis i
• Research Area: Cancer Biology, Infectious Disease & Virology, Neu
• Note: For research use only
• NCBI: NP_000563.1
• Expiration Date: 12 months from date of receipt.

To a sandwich ELISA (assuming 100 ul/well), a concentration of 4.0-8.0 ug/ml of this antibody will detect at least 1600 pg/ml of recombinant murine IL-10 when used with biotinylated antigen affinity purified anti-murine IL-10 (orb1272494) as the detection antibody at a concentration of approximately 0.5-1.0 ug/ml.

To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.

To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.