Histone H2B rabbit pAb

• Catalog Number: orb765402
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Histone H2B
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Monkey, Mouse
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H2B. AA range:10-59
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 14
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-H2BFS antibody, anti-Histone H2B type F-S ant
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using Histone H2B Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Western Blot analysis of COS7 cells using Histone H2B Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Immunofluorescence analysis of HeLa cells, using Histone H2B Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2B Antibody. The picture on the right is blocked with the synthesized peptide.