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Item 1 of 4
Item 1 of 4
HIST1H2BJ Antibody (Center)
Catalog Number: orb1938754
| Catalog Number | orb1938754 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB28555 |
| Tested applications | FC, IHC-P, WB |
| Predicted Reactivity | Mouse, Other, Rat, Zebrafish |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 13904 Da |
| Target | This HIST1H2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-86 amino acids from the Central region of human HIST1H2BJ. |
| UniProt ID | P06899 |
| NCBI | NP_066402.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Histone H2B type 1-J, Histone H2B1, Histone H2Br, Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
HIST1H2B Antibody (Center) western blot analysis in CEM, HL-60, NCI-H460 cell line lysates (35 ug/lane). This demonstrates the HIST1H2B antibody detected the HIST1H2B protein (arrow).
HIST1H2B Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
HIST1H2B antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIST1H2B antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of lysates from ZF4 cell line, Zebrafish, zebra fish muscle tissue lysate (from left to right), using HIST1H2BJ Antibody (Center). diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary Antibody. Lysates at 35 ug per lane.
