HIST1H2BJ Antibody (Center)

SKU: orb1938754

Description

HIST1H2BJ Antibody (Center)

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Bovine, Gallus, Monkey, Mouse, Rat, Xenopus, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 13904 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

HIST1H2B Antibody (Center) western blot analysis in CEM, HL-60, NCI-H460 cell line lysates (35 ug/lane). This demonstrates the HIST1H2B antibody detected the HIST1H2B protein (arrow).

HIST1H2B Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

HIST1H2B antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIST1H2B antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of lysates from ZF4 cell line, Zebrafish, zebra fish muscle tissue lysate (from left to right), using HIST1H2BJ Antibody (Center). diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary Antibody. Lysates at 35 ug per lane.

Quick Database Links

UniProt

P06899