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Catalog Number | orb769515 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Histone H2B (Acetyl Lys15) |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human Histone H2B around the acetylated site of Lys15. AA range:1-50 |
Concentration | 1 mg/ml |
Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
Conjugation | Unconjugated |
MW | 20 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-H2BFS antibody, anti-Histone H2B type F-S ant Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot analysis of 3T3 cells using Acetyl-Histone H2B (K15) Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.
Immunofluorescence analysis of HeLa cells, using Histone H2B (Acetyl-Lys15) Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2B (Acetyl-Lys15) Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from Raw264.7 cells, treated with TSA 400nM 24h, using Histone H2B (Acetyl-Lys15) Antibody. The lane on the right is blocked with the synthesized peptide.
ICC, IF, IHC-Fr, IHC-P, WB | |
Human, Mouse | |
Polyclonal | |
Unconjugated |