Histone H2B (Acetyl Lys15) rabbit pAb

• Catalog Number: orb769515
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Histone H2B (Acetyl Lys15)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H2B around the acetylated site of Lys15. AA range:1-50
• MW: 20
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-H2BFS antibody, anti-Histone H2B type F-S ant
• Note: For research use only

Western Blot analysis of 3T3 cells using Acetyl-Histone H2B (K15) Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.

Immunofluorescence analysis of HeLa cells, using Histone H2B (Acetyl-Lys15) Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2B (Acetyl-Lys15) Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Raw264.7 cells, treated with TSA 400nM 24h, using Histone H2B (Acetyl-Lys15) Antibody. The lane on the right is blocked with the synthesized peptide.