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| Catalog Number | orb769514 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to Acetyl Histone H2B (K12) |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, IHC-P, WB |
| Reactivity | Human, Monkey, Mouse |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Histone H2B around the acetylated site of Lys12. AA range:10-59 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 14 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-H2BFS antibody, anti-Histone H2B type F-S ant Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of HeLa cells, using Histone H2B (Acetyl-Lys12) Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Histone H2B (Acetyl-Lys12) Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from COS7 cells, treated with TSA 400nM 24h, using Histone H2B (Acetyl-Lys12) Antibody. The lane on the right is blocked with the synthesized peptide.
