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| Catalog Number | orb18651 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to PEBP1 |
| Target | PEBP1 |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Mouse, Rat |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | DYVPKLYEQLSGK |
| RRID | AB_10749324 |
| MW | 21.1 |
| Tested applications | ELISA, IHC, WB |
| Dilution range | ELISA: 1:32000, WB: 0.5-2 μg/ml, IHC-P: 5 μg/ml |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Brain (Cortex) shows staining near sectect nuclei. Recommended concentration, 1-2ug/ml.WB: Approx 22kDa band observed in lysates of cell lines HEK293, A549 and HepG2 (calculated MW of 21.1kDa according to NP_002558.1). Recommended concentration: 0.01-0.03 μg/ml. A customer has reported the 22kDa in Rat Hippocampus using this antibody. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti PEBP1 antibody, anti phosphatidylethanolamine Read more... |
| Research Area | Epigenetics |
| Note | For research use only |
| Entrez | 5037 |

Western blot analysis of Human Cerebellum (Lane 1) and Mouse Testes (Lane 2) lysate using PEBP1 antibody

Immunohistochemical staining of Human Cortex using PEBP1 antibody

Immunohistochemical staining of Human Prostate using PEBP1 antibody

Western blot analysis of NIH-3T3 (Lane 1) , HEK293 (Lane 2) and HepG2 (Lane 3) lysate using PEBP1 antibody

Western blot analysis of A549 lysate using PEBP1 antibody
ELISA, FC, ICC, IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Human | |
0.16-10 ng/mL | |
0.058 ng/mL |
Rat | |
0.16-10 ng/mL | |
0.059 ng/mL |
Mouse | |
0.16-10 ng/mL | |
0.054 ng/mL |
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