PEBP1/RKIP (Internal) Antibody

• Catalog Number: orb19343
• Category: Antibodies
• Description: Goat polyclonal antibody to PEBP1
• Target: PEBP1 / RKIP (Internal)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DPDAPSRKDPKYRE
• RRID: AB_10755704
• MW: 21.1
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.01-0.03 μg/ml, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Adrenal Gland shows strong stinaing of secretory cells. Recommended concentration, 2-5ug/ml.WB: Approx 21kDa band observed in Human Prostate lysates (calculated MW of 21.1kDa according to NP_002558.1). Recommended concentration: 0.01-0.03 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PEBP1 antibody, anti RKIP antibody, anti phos
• Note: For research use only
• Entrez: 5037
• Expiration Date: 12 months from date of receipt.

orb19343 (0.01ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19343 (2.5ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemical staining of Human Kidney using PEBP1 antibody