GFAP Antibody

• Catalog Number: orb19324
• Category: Antibodies
• Description: Goat polyclonal to GFAP
• Target: GFAP
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DGEVIKESKQEHKD
• RRID: AB_10748765
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:128000, WB: 0.001-0.1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Cerebellum shows specific staining of astrocytes. Recommended concentration, 2-4µg/ml.WB: Sandwich-type ELISA with increasing amount of recombinant GFAP captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml. Please note this data was obtained using a previous batch.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti GFAP antibody, anti glial fibrillary acidic p
• Research Area: Epigenetics, Neuroscience, Stem Cells
• Note: For research use only
• Entrez: 2670
• Expiration Date: 12 months from date of receipt.

orb19324 (0.001µg/ml) staining of Human Cerebellum (A), (0.1µg/ml) Mouse Brain (B) and (0.03µg/ml) Rat Brain (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Western blot analysis of Human Brain (Cerebellum) (A), Mouse (B) and Rat (C) Brain lysate using GFAP antibody