GFAP antibody

• Catalog Number: orb10706
• Category: Antibodies
• Description: GFAP antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GFAP. Please contact us for the exact immunogen sequence. The peptide is available as orb374725.
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Dilution range: WB: 1:100-1000, IHC-P: 1:100-1000, IF/ICC: 1:100-1000 (based on 0.5mg/ml)
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated
• MW: 50 kDa
• Target: GFAP
• Entrez: 2670
• UniProt ID: P14136, Q28115, P03995
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Alternative names: anti-Glial Fibrillary Acidic Protein antibody, ant
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Masoumeh Rahmatinia 1, Anoushiravan Mohseni-Bandpei 1, Fariba Khodagholi 2, Mohammad-Amin Abdollahifar 3, Mehdi Amouei Torkmahalleh 4, Meysam Hassani Moghaddam 5, Philip K Hopke 6, Ehsan Ghavimehr 7, Shahriyar Bazzazpour 8, Abbas Shahsavani 9 Exposure to different PM2.5 extracts induces gliosis and changes behavior in male rats similar to autism spectrum disorders features Environ Pollut, 340, 122804 (2024)

PubMed: 37907193

Applications

IHC

Reactivity

Rat

Western blot analysis of rat brain (lane 1), rat spinal cord (lane 2), rat lung (lane 3), mouse pancreas (lane 4), rat liver (lane 5), U251 cells (lane 6) using GFAP antibody (1 ug/ml)

Immunofluorescence analysis of mouse lung tissue using GFAP antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat pancreas tissue using GFAP antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded mouse brain tissue using GFAP antibody (2.5 ug/ml)

Immunohistochemical staining of rat pancreas tissue using anti-GFAP (2.5 ug/ml)

IHC-P staining of mouse brain tissue using GFAP antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded mouse lung tissue using anti-GFAP (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat spinal cord tissue using GFAP antibody (2.5 ug/ml)

IHC-P staining of mouse skin tissue using anti-GFAP (2.5 ug/ml)

IHC-P image of mouse skin tissue using GFAP antibody (2.5 ug/ml)

IHC-P staining of rat pancreas tissue using anti-GFAP (2.5 ug/ml)

IF image of mouse lung tissue using GFAP antibody (2.5 ug/ml)

Immunohistochemical staining of mouse brain tissue using anti-GFAP (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded mouse lung tissue using anti-GFAP (2.5 ug/ml)

WB analysis of rat brain (lane 1), rat spinal cord (lane 2), rat lung (lane 3), mouse pancreas (lane 4), rat liver (lane 5), U251 cells (lane 6) using GFAP antibody (1 ug/ml)