Glial Fibrillary Acidic Protein (GFAP) Antibody

SKU: orb319550

Description

Our Anti-Glial Fibrillary Acidic Protein (GFAP) primary antibody from Biorbyt is chicken polyclonal. It detects bovine, human, mouse, and rat Glial Fibrillary Acidic Protein (GFAP) and is total IgY fraction.

Research Area

Disease Biomarkers, Neuroscience

Images & Validation

• Tested Applications: ELISA, ICC, IHC, WB
• Dilution Range: WB: 1:10,000, IHC: 1:1000-1:5000, ICC: 1:1000-1:5000
• Reactivity: Bovine, Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Gallus
• Clonality: Polyclonal
• Isotype: IgY
• Immunogen: Recombinant human GFAP.
• Target: Glial Fibrillary Acidic Protein (GFAP)
• Molecular Weight: 50
• Purification: Total IgY fraction
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS + 10 mM NaN3.
• Concentration: 30 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

wu:fb34h11 antibody, ALXDRD antibody, cb345 antibody, etID36982.3 antibody, FLJ42474 antibody, FLJ45472 antibody, GFAP antibody, GFAP_HUMAN antibody, gfapl antibody, Glial fibrillary acidic protein antibody, Intermediate filament protein antibody, wu:fk42c12 antibody, xx:af506734 antibody, zgc:110485 antibody

Immunofluorescence of a section of mouse hippocampus colabeled with Anti-GFAP (Cat. orb319550, green, 1:5000) and Anti-FOX3 (red). The Anti-FOX3 labels the nuclei and proximal perikarya of neurons while the Anti-GFAP labels a network of astroglial cells. The blue is DAPI staining of nuclear DNA.

Immunofluorescence of a section of mouse prefrontal cortex labeled with Anti-GFAP (Cat. orb319550, red, 1:1000).

Immunofluorescence of a section of rat cerebellum showing specific labeling of MeCP2 (Cat. orb55908, 1:1000, red) in nuclei of neurons and specific labeling of GFAP (Cat. orb319550, 1:5000, green) in the network of astroglial cells and projections of Bergmann glia, and Hoechst staining of nuclear DNA.

Immunofluorescence of a section of rat frontal cortex showing labeling of Neurofilament-L (Cat. orb319586, 1:500, red) and labeling of GFAP (Cat. orb319550 , 1:5000, green). The anti-NFL antibody labels cell bodies and processes of pyramidal neurons, as well as dendrites and axons of other neuronal cells. While the anti-GFAP antibody labels the network of glial cells.

Immunolabeling of cultured rat neurons showing strong nuclear and distal cytoplasmic labeling with anti-FOX3 (Cat. orb613671, 1:1000, red). The complete absence of astrocyte staining is shown using anti-GFAP (Cat. orb319550, 1:1000, green) and nuclear staining was done with DAPI (blue).

Immunolabeling of E20 rat mixed neuron and glial cultured cells labeled with anti-neuron specific enolase antibody (Cat. orb319615, red, 1:500) and anti-GFAP antibody (Cat. orb319550, green, 1:5000). The blue is Hoechst stain of nuclear DNA. Anti-NSE labels protein expressed in neuronal cells and anti-GFAP labels protein expressed in intermediate filaments in astrocytic and other glial cells.

Immunolabeling of mixed neuron and glia cultures where astrocytes are strongly and specifically labeled with Anti-GFAP (Cat. orb319550, 1:1000, red), and nuclear staining with DAPI (blue).

Immunostaining of E20 rat cortical neuron/glial cell culture stained with anti-vimentin (Cat. orb319708, red, 1:2000) and anti-GFAP antibody (Cat. orb319550, green, 1:5000). The blue is DAPI staining nuclear DNA. Vimentin is expressed alone in fibroblastic and developing cells and appear red. The astrocytes that appear red express only GFAP, while the golden yellow and yellow astrocytes express GFAP and Vimentin.

Western blot of rat cortical lysate showing specific immunolabeling of the ~50 kDa GFAP protein.

Quick Database Links

Gene Symbol

Glial Fibrillary Acidic Protein (GFAP)

UniProt

P14136