GFAP Recombinant Rabbit Monoclonal Antibody

SKU: orb1152219

Description

GFAP Recombinant Rabbit Monoclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:1000-50000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 3F4
• Immunogen: A synthesized peptide derived from human GFAP (1-41aa)
• Target: GFAP
• Molecular Weight: 48 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Astrocyte; FLJ45472; Glial Fibrillary Acidic Protein; Intermediate filament protein; GFAP_HUMAN.

Cell line: Mouse primary astrocyte, Fixation: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:50, Primary Ab incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1152219.

Cell line: Neuro-2a (Negative cell line), Fixation: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:50, Primary Ab incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: No staining on orb1152219.

Paraformaldehyde-fixed, paraffin embedded (MOUSE brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (orb1152219) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (orb1152219) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (orb1152219) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (orb1152219) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Rat Cerebrum tissue lysates, Lane 2: Rat Cerebellum tissue lysates, Lane 3: Human U251 cell lysates, Primary: Anti-GFAP (orb1152219) at 1/10000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kDa, Observed band size: 48 kDa.

Quick Database Links

Gene Symbol

GFAP

UniProt

P14136