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Goat anti-GAD1 (isoform GAD67) Antibody

Catalog Number: orb20081

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb20081
CategoryAntibodies
DescriptionGoat polyclonal antibody to GAD1
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, FC, IF, WB
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
Dilution rangeELISA: 1:32000, WB: 0.5-1.5 μg/ml
ConjugationUnconjugated
MW66.9
TargetGAD1 (isoform GAD67)
Entrez2571
Protein SequencePDSPQRREKLHK
RRIDAB_10752062
StorageAliquot and store at -20°C. Minimize freezing and thawing.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesanti GAD1 antibody, anti glutamate decarboxylase 1
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NoteFor research use only
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 70kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 66.9kDa according to Human NP_000808.2 and 66.6kDa according to Mouse NP_032103.2). Recommended concentration: 0.3-1 μg/ml.
Expiration Date12 months from date of receipt.
Goat anti-GAD1 (isoform GAD67) Antibody

orb20081 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Goat anti-GAD1 (isoform GAD67) Antibody

orb20081 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Goat anti-GAD1 (isoform GAD67) Antibody

orb20081 (1µg/ml) staining of Human Cerebellum (A) and Mouse Brain (B) and (0.3ug/ml) Rat Brain (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Goat anti-GAD1 (isoform GAD67) Antibody

orb20081 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Goat anti-GAD1 (isoform GAD67) Antibody

Western blot analysis of Human Brain lysate using GAD1 antibody

Goat anti-GAD1 (isoform GAD67) Antibody

Immunohistochemical staining of Human Cerebellum using GAD1 antibody

  • Goat anti-GAD25 / GAD67 Antibody [orb125196]

    ELISA,  WB

    Bovine, Canine, Human, Mouse, Porcine, Rat

    Goat

    Polyclonal

    Unconjugated

    100 μg