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Catalog Number | orb20081 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to GAD1 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IF, WB |
Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
Dilution range | ELISA: 1:32000, WB: 0.5-1.5 μg/ml |
Conjugation | Unconjugated |
MW | 66.9 |
Target | GAD1 (isoform GAD67) |
Entrez | 2571 |
Protein Sequence | PDSPQRREKLHK |
RRID | AB_10752062 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti GAD1 antibody, anti glutamate decarboxylase 1 Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 70kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 66.9kDa according to Human NP_000808.2 and 66.6kDa according to Mouse NP_032103.2). Recommended concentration: 0.3-1 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb20081 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
orb20081 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
orb20081 (1µg/ml) staining of Human Cerebellum (A) and Mouse Brain (B) and (0.3ug/ml) Rat Brain (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
orb20081 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of Human Brain lysate using GAD1 antibody
Immunohistochemical staining of Human Cerebellum using GAD1 antibody
ELISA, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |