Anti-GAD1 Antibody

• Catalog Number: orb1289742
• Category: Antibodies
• Description: Rabbit Polyclonal antibody to GAD1
• Target: GAD1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: Recombinant fusion protein of human GAD1. The exact sequence is proprietary.
• UniProt ID: P48318, Q99259, P18088
• Tested applications: IF, IH, WB
• Dilution range: WB: 1:500-1:1000, IH: 1:50-1:200, IF: 1:50-1:200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GAD; GAD67; Glutamate decarboxylase 1; 67 kDa glut
• Note: For research use only
• Entrez: 2571, 14415, 24379

Western blot analysis of GAD1 expression in Jurkat (A), mouse pancreas (B) whole cell lysates. (Predicted band size: 25; 47; 66 kD; Observed band size: 67 kD)

Immunohistochemical analysis of GAD1 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of GAD1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.