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GAD67 Rabbit Polyclonal Antibody

Catalog Number: orb10681

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb10681
CategoryAntibodies
DescriptionGAD67 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityRat
ReactivityHuman, Mouse
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human GAD67 (455-594/594aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW67 kDa
TargetGAD1
UniProt IDQ99259
RRIDAB_10762858
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesglutamate decarboxylase 67; decarboxylase 1; 67 kD
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NoteFor research use only
Expiration Date12 months from date of receipt.
GAD67 Rabbit Polyclonal Antibody

Blank control: K562. Primary Antibody (green line): Rabbit Anti-GAD67 antibody (orb10681), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

GAD67 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GAD67) Polyclonal Antibody, Unconjugated (orb10681) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

GAD67 Rabbit Polyclonal Antibody

Sample: Raji (Human) Cell Lysate at 40 ug, Primary: Anti-GAD67 (orb10681) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 67 kD, Observed band size: 67 kD.

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