GAD1/2 Rabbit Polyclonal Antibody

SKU: orb213964

Description

The GAD1/2 Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GAD1/2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics, Neuroscience

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1-500-1-1000, IHC-P: 1-100-1-200
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GAD1/2. The exact sequence is proprietary.
• Target: GAD1; GAD2
• Molecular Weight: 66 kDa
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GAD1; GAD; GAD67; Glutamate decarboxylase 1; 67 kDa glutamic acid decarboxylase; GAD-67; Glutamate decarboxylase 67 kDa isoform; GAD2; GAD65; Glutamate decarboxylase 2; 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform

Immunofluorescent analysis of GAD1/2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Immunohistochemical analysis of paraffin-embedded mouse heart using GAD1/2 Antibody orb213964 with dilution at 1:100.

Western blot analysis of mouse kidney (1), rat brain (2), mouse brain (3) using GAD1/2 Antibody orb213964 with dilution at 1:1000.

Quick Database Links

Gene Symbol

GAD1; GAD2

UniProt

Q99259, P48318, P48320, Q05329

NCBI Gene ID

14417, 14415, 2571, 2572