beta-2-microglobulin Antibody

SKU: orb12337

Description

Goat polyclonal to B2M.

Images & Validation

• Tested Applications: ELISA, FC, IF
• Dilution Range: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary testing showed a band at approx. 12-13kDa in Human Spleen lysate and in Jurkat, U251, U2OS, U937 and HeLa cell lysate after 0.3-1µg/ml antibody staining (calculated MW of 13.7kDa according to NP_004039.1). Primary incubation 1 hour at room temperature. Immunofluorescence: Strong expression of the protein seen in the plasma membranes and cytoplasm of HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Reactivity: Human
• Predicted Reactivity: Bovine

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: beta-2-microglobulin
• Protein Sequence: QRTPKIQVYSRHP
• Molecular Weight: 13.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti B2M antibody

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Quick Database Links

Gene Symbol

beta-2-microglobulin

NCBI Gene ID

567