beta-2-microglobulin Antibody

• Catalog Number: orb12337
• Category: Antibodies
• Description: Goat polyclonal to B2M.
• Target: beta-2-microglobulin
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QRTPKIQVYSRHP
• MW: 13.7
• Tested applications: ELISA, FC, IF
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary testing showed a band at approx. 12-13kDa in Human Spleen lysate and in Jurkat, U251, U2OS, U937 and HeLa cell lysate after 0.3-1µg/ml antibody staining (calculated MW of 13.7kDa according to NP_004039.1). Primary incubation 1 hour at room temperature. Immunofluorescence: Strong expression of the protein seen in the plasma membranes and cytoplasm of HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti B2M antibody
• Note: For research use only
• Entrez: 567

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.