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Beta-2 Microglobulin Antibody

Catalog Number: orb749756

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb749756
CategoryAntibodies
DescriptionRecognizes a protein of 12kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.
Species/HostMouse
ClonalityMonoclonal
Clone NumberB2M/961
Tested applicationsFACS, IF, IHC-P, WB
ReactivityHuman
IsotypeMouse IgG2b, kappa
ImmunogenFull length recombinant human protein was used as the immunogen for the Beta-2 Microglobulin antibody.
Antibody TypePrimary Antibody
Dilution rangeFlow cytometry: 1-2ug/million cells,Immunofluorescence: 1-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis Beta-2 Microglobulin antibody is available for research use only.
UniProt IDP61769
StorageStore the Beta-2 Microglobulin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the Beta-2 Microglobulin antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
Beta-2 Microglobulin Antibody

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).

Beta-2 Microglobulin Antibody

IHC: Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).

Beta-2 Microglobulin Antibody

IHC: Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).

Beta-2 Microglobulin Antibody

IHC: Formalin-fixed, paraffin-embedded human renal carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).

Beta-2 Microglobulin Antibody

IHC: Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).

Beta-2 Microglobulin Antibody

IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).

Beta-2 Microglobulin Antibody

IHC: Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).

Beta-2 Microglobulin Antibody

Immunofluorescent staining of human HeLa cells with Beta-2 Microglobulin antibody (green, clone B2M/961) and Reddot nuclear stain (red).

Beta-2 Microglobulin Antibody

Western blot testing of human Raji cell lysate with Beta-2-Microglobulin antibody (clone B2M/961).

Beta-2 Microglobulin Antibody

Flow cytometry testing of human HeLa cells with Beta-2 Microglobulin antibody (clone B2M/961); Red = isotype control, Blue = Beta-2 Microglobulin antibody.

Beta-2 Microglobulin Antibody

IHC: Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).

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