Beta-2 Microglobulin Antibody

SKU: orb2639105

Description

Recognizes a protein of 12kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Beta-2 Microglobulin antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: B2M/961
• Immunogen: Full length recombinant human protein was used as the immunogen for the Beta-2 Microglobulin antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).

IHC: Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).

IHC: Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).

IHC: Formalin-fixed, paraffin-embedded human renal carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).

IHC: Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).

IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).

IHC: Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).

Immunofluorescent staining of human HeLa cells with Beta-2 Microglobulin antibody (green, clone B2M/961) and Reddot nuclear stain (red).

Western blot testing of human Raji cell lysate with Beta-2-Microglobulin antibody (clone B2M/961).

Flow cytometry testing of human HeLa cells with Beta-2 Microglobulin antibody (clone B2M/961); Red = isotype control, Blue = Beta-2 Microglobulin antibody.

IHC: Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).

Quick Database Links

UniProt

P61769