ATG5 Antibody

SKU: orb20003

Description

Goat polyclonal antibody to ATG5

Research Area

Apoptotic, Autophagic, Cancer, Cardiovascular Disease, Epigenetics, Immunology, Infectious Diseases, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA
• Dilution Range: ELISA: 1:64000, WB: 1 μg/ml
• Predicted Reactivity: Bovine, Human, Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave an approx. 38 kDa band in NIH3T3 lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.4 kDa according to NP_004840.1. The 38 kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ATG5
• Protein Sequence: SQVINEMQKKDHKQ
• Molecular Weight: 32.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ATG5 antibody, anti ATG5 autophagy related 5 homolog (S. cerevisiae) antibody, anti APG5 antibody, anti APG5L antibody, anti ASP antibody, anti hAPG5 antibody, anti APG5 (autophagy 5, S. cerevisiae)-like antibody, anti APG5 autophagy 5-like antibody, anti ATG5 autophagy related 5-like antibody, anti OTTHUMP00000040507 antibody, anti apoptosis specific protein antibody

Quick Database Links

Gene Symbol

ATG5

NCBI Gene ID

9474