Goat Anti-AIF1/IBA1 isoform 1 and 3 (Human reactivity only) Antibody

• Catalog Number: orb1994921
• Category: Antibodies
• Description: Goat Anti-AIF1/IBA1 isoform 1 and 3 (Human reactivity only) Antibody
• Species/Host: Goat
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: Peptide with sequence C-TGPPAKKAISELP.., from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3.
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx. 18kDa band observed in Human Frontal Cortex lysates (calculated MW of 16.7kDa according to NP_001614.). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
• Conjugation: Unconjugated
• NCBI: NP_001614.3, NP_116573.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
• Alternative names: AIF1, IBA1, allograft inflammatory factor 1, AIF-1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

4 µg/ml staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

2 ug/ml staining of Human Frontal Cortex lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.