Goat Anti-AIF1/IBA1 isoform 1 and 3 (Mouse reactivity only) Antibody

• Catalog Number: orb1994920
• Category: Antibodies
• Description: Goat Anti-AIF1/IBA1 isoform 1 and 3 (Mouse reactivity only) Antibody
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: Peptide with sequence C-TGPPAKKAISELP,, from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3.
• Tested applications: ELISA, WB
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 16kDa band observed in Mouse Brain lysates (calculated MW of 16.9kDa according to NP_062340.1 ). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: AIF1, IBA1, allograft inflammatory factor 1, AIF-1
• Note: For research use only
• NCBI: NP_001614.3, NP_116573.1
• Expiration Date: 12 months from date of receipt.

0.3 µg/mL staining of Mouse Brain lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.