GFAP (Astrocyte & Neural Stem Cell Marker) Antibody

• Catalog Number: orb1410371
• Category: Antibodies
• Description: Mouse monoclonal antibody to GFAP (Astrocyte & Neural Stem Cell Marker)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: GFAP/4450
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Isotype: IgG2b
• Immunogen: Recombinant full-length human GFAP protein
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: ~50kDa
• Target: GFAP
• Entrez: 2670
• UniProt ID: P14136
• Expression System: HEK293 cells
• Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: Astrocyte or Intermediate Filament Protein, Glial
• Note: For research use only
• Application notes: Positive Control: Human brain or astrocytoma
• Expiration Date: 12 months from date of receipt.