GFAP (Astrocyte & Neural Stem Cell Marker) Antibody

• Catalog Number: orb1410271
• Category: Antibodies
• Description: Mouse monoclonal antibody to GFAP (Astrocyte & Neural Stem Cell Marker)
• Target: GFAP
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2c
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Purification: Protein A/G
• Immunogen: Recombinant fragment (around aa 150-250) of human GFAP protein (exact sequence is proprietary)
• UniProt ID: P14136
• MW: ~50kDa
• Tested applications: IHC, WB
• Clone Number: GFAP/6880
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Astrocyte or Intermediate Filament Protein, Glial
• Research Area: Cardiovascular Research, Neuroscience, Signal Tran
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Analysis of Protein Array containing more than 19000 full-length human proteins using Monospecific Mouse Monoclonal Antibody to GFAP (GFAP/6880). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP Mouse Monoclonal Antibody (GFAP/6880). HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.

Formalin-fixed, paraffin-embedded human cerebral cortex stained with GFAP Mouse Monoclonal Antibody (GFAP/6880). HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.

IHC analysis of FFPE human heart (RNA expression: NX 4.0). Negative tissue control using GFAP/6880 at 2 ug/ml in PBS for 30 min RT. HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.

SDS-PAGE Analysis Purified GFAP Mouse Monoclonal Antibody (GFAP/6880). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of human brain tissue lysate using GFAP Mouse Monoclonal Antibody (GFAP/6880).