GFAP Antibody

SKU: orb1409994

Description

Mouse monoclonal antibody to GFAP (Astrocyte & Neural Stem Cell Marker)

Research Area

Cardiovascular Research, Neuroscience, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: GFAP/6884
• Immunogen: Recombinant fragment (around aa 150-250) of human GFAP protein (exact sequence is proprietary)
• Target: GFAP
• Source/Expression System: Bioreactor
• Molecular Weight: ~50kDa
• Purification: Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Disclaimer: For research use only

Alternative Names

Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)

Analysis of Protein Array containing more than 19000 full-length human proteins using Monospecific Mouse Monoclonal Antibody to GFAP (GFAP/6884). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human brain stained with GFAP Mouse Monoclonal Antibody (GFAP/6884). HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.

Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP Mouse Monoclonal Antibody (GFAP/6884). HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.

SDS-PAGE Analysis of Purified GFAP Mouse Monoclonal Antibody (GFAP/6884). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of human brain tissue lysate using GFAP Mouse Monoclonal Antibody (GFAP/6884).

Western blot analysis of human brain tissue lysate using GFAP Mouse Monoclonal Antibody (GFAP/6884).

Quick Database Links

Gene Symbol

GFAP

UniProt

P14136