GFAP Mouse Monoclonal Antibody

SKU: orb627313

Description

Mouse monoclonal antibody to GFAP

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC, IP, WB
• Dilution Range: WB: 1:5000-1:50000; IP: 1:500-1:5000; IHC: 1:500-1:10000; IF: 1:50-1:500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Immunogen: glial fibrillary acidic protein
• Target: GFAP
• Molecular Weight: 45-50 kDa
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Protein A+G purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: liquid
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IP Result of anti-GFAP (IP: orb627313, 5 ug; Detection: orb627313 1:1000) with mouse brain tissue lysate 2640 ug.

U-251 cellswere subjected to SDS PAGE followed by western blot with orb627313 (GFAP antibody) at dilution of 1:10000.

Immunohistochemistry of paraffin-embedded human brain tissue slide using orb627313 (GFAP Antibody) at dilution of 1:500 Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

Quick Database Links

Gene Symbol

GFAP

UniProt

P14136