GFAP antibody

• Catalog Number: orb1338576
• Category: Antibodies
• Description: Mouse monoclonal antibody to GFAP
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: OTI2C4
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG2b
• Immunogen: Full length human recombinant protein of human GFAP (NP_002046) produced in HEK293T cell.
• Dilution range: WB 1:2000, IHC 1:50, IF 1:100
• Conjugation: Unconjugated
• MW: 49.9 kDa
• Target: GFAP
• UniProt ID: P14136
• NCBI: NM_002055
• Note: For research use only
• Application notes: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
• Expiration Date: 12 months from date of receipt.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GFAP (RC204548, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GFAP.

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-GFAP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min)

Anti-GFAP mouse monoclonal antibody (TA500335) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GFAP.