GFAP Antibody / Glial Fibrillary Acidic Protein

• Catalog Number: orb750205
• Category: Antibodies
• Description: Glial Fibrillary Acidic Protein is specifically found in astroglia, making it a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Ab to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM248
• Tested applications: FACS, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: GFAP isolated from pig spinal cord was used as the immunogen for this Glial Fibrillary Acidic Protein antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1) (2)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Glial Fibrillary Acidic Protein antibody is available for research use only.
• UniProt ID: P14136
• Storage: Store the Glial Fibrillary Acidic Protein antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human schwanoma stained with Glial Fibrillary Acidic Protein antibody (clone SPM248).

Western blot testing of human T98G cell lysate with Glial Fibrillary Acidic Protein antibody (clone SPM248). Expected molecular weight 50~55 kDa.

Flow cytometry testing of permeabilized human T98G cells with Glial Fibrillary Acidic Protein antibody (clone SPM248); Red=isotype control, Blue= Glial Fibrillary Acidic Protein antibody.

SDS-PAGE analysis of purified, BSA-free Glial Fibrillary Acidic Protein antibody (clone SPM248) as confirmation of integrity and purity.