ERK 3 rabbit pAb

• Catalog Number: orb769692
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ERK 3
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Immunogen: The antiserum was produced against synthesized peptide derived from human p97 MAPK. AA range:461-510
• MW: 85
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-MAPK6 antibody, anti-ERK3 antibody, anti-PRKM
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Hela Lysate, antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunofluorescence analysis of MCF7 cells, using p97 MAPK Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from K562 cells, using p97 MAPK Antibody. The lane on the right is blocked with the synthesized peptide.