You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1937566 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB31616 |
Tested applications | FC, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 147437 Da |
Target | This MAP3K15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 315-342 amino acids from the N-terminal region of human MAP3K15. |
UniProt ID | Q6ZN16 |
NCBI | NP_001001671.3 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Mitogen-activated protein kinase kinase kinase 15, Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-MAP3K15 Antibody (N-term) at 1:1000 dilution + human fetal brain lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 147 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
MAP3K15 Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35 ug/lane). This demonstrates the MAP3K15 antibody detected the MAP3K15 protein (arrow).
MAP3K15 Antibody (N-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
MAP3K15 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MAP3K15 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.