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MAPK12 Antibody (Center)

Catalog Number: orb1929463

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb1929463
CategoryAntibodies
DescriptionPurified Rabbit Polyclonal Antibody (Pab)
Species/HostRabbit
ClonalityPolyclonal
Clone NumberRB03195/03196
Tested applicationsIHC-P, WB
ReactivityHuman, Mouse
IsotypeRabbit IgG
Antibody TypePrimary Antibody
Dilution rangeWB: 1:1000, WB: 1:1000, WB: 1:2000, IHC-P: 1:50~100
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
ConjugationUnconjugated
MW41940 Da
TargetThis MAPK12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 339-367 amino acids from the Central region of human MAPK12.
UniProt IDP53778
NCBINP_002960.2
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesMitogen-activated protein kinase 12, MAP kinase 12
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NoteFor research use only
Expiration Date12 months from date of receipt.
MAPK12 Antibody (Center)

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

MAPK12 Antibody (Center)

Western blot analysis of p38 gamma (MAPK12) Antibody (Center) in mouse lung tissue lysates (35 ug/lane). MAPK12 (arrow) was detected using the purified Pab.

MAPK12 Antibody (Center)

Western blot analysis of MAPK12 (arrow) using rabbit polyclonal hMAPK12-P354. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAPK12 gene.

MAPK12 Antibody (Center)

All lanes: Anti-MAPK12 Antibody (P354) at 1:2000 dilution. Lane 1: Hela whole cell lysates. Lane 2: Jurkat whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

  • MAPK12 Antibody (Center) [orb1166403]

    IHC-P,  WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 30 μl